Ovariectomy exacerbates glycerol-induced acute kidney injury in rats

Authors

DOI:

https://doi.org/10.12662/2317-3076jhbs.v10i1.4121.p1-8.2022

Keywords:

Ovariectomy, Glycerol. Rhabdomyolysis, Acute Kidney Injury, Inflammation

Abstract

Objective: This study aimed to evaluate the effects of ovariectomy on glycerol-induced renal changes in rats. Methods: Twenty-four female Wistar rats were submitted to ovariectomized (OVX) or sham surgery. One week after surgery, the animals received an intramuscular injection (8ml/kg) of 50% glycerol or saline (0.15 M) solution. These animals were divided into the following groups (n=6 per group): Sham, sham-operated female rats injected with saline; OVX, ovariectomized female rats injected with saline; Sham+Gly, sham-operated female rats injected with glycerol; OVX+Gly, ovariectomized female rats
injected with glycerol. All rats were euthanized 3 days after the injections and the kidneys were removed for histological and immunohistochemical studies. Blood and urine samples were also collected for renal function studies. Results: The OVX+Gly group presented higher creatinine serum levels, as well as
greater fractional excretion of sodium and urinary flow than the Sham+Gly group. Histological lesions and tubulointerstitial staining for macrophages, nuclear factor-kappa B, and nitrotyrosine were more pronounced in the renal cortex of the OVX+Gly group compared to the Sham+Gly group. Conclusion:
We conclude that ovariectomy aggravated changes in renal function and structure in glycerol-induced acute kidney injury by the intensification of the proinflammatory tissue response.

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Published

2022-11-03

How to Cite

1.
Coimbra-Campos LM de C, Souza SI de, Amaral LS de B, Silva F de A, Gusmão ACM de M, Coimbra TM, et al. Ovariectomy exacerbates glycerol-induced acute kidney injury in rats. J Health Biol Sci. [Internet]. 2022 Nov. 3 [cited 2024 Dec. 23];10(1):1-8. Available from: https://unichristus.emnuvens.com.br/jhbs/article/view/4121

Issue

Vol. 10 No. 1 (2022): Journal of Health and Biological Sciences

Section

Original articles

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